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Our project has generated a number of strains in which GFP expression
was not detected, or expression was lost before the strain could be
fully analyzed. There are a number of possible causes:
1)
germline/gene silencing (Kelly et al 1997; Reviewed in Seydoux and
Schedl 2001),
2) absence of promoter::GFP in the heritable arrays,
3)
conditional gene expression (environmental, male, dauer etc.),
4)
failure to capture the entire transcription control element,
5) the
promoter is within an operon, etc. Therefore, the following list is
merely useful as a comparison baseline for other projects when they use
these promoter regions. No conclusions can be drawn from this data.
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